Proinsulin ELISA

Measurement range: 3.3 - 130 pmol/L (0.03 - 1.18 µg/L)

l  Measures total proinsulin

l  CE/IVD labeled on selected markets

l  Highly specific with no cross-reactivity to human insulin or C-peptide

l  Calibrated against International Reference Reagent IRR 84/611 for human proinsulin

l  Serum controls available: 10-1134-01 (US only) or 10-1164-01

Description

The Proinsulin ELISA is a method for the quantitative determination of human proinsulin in serum or plasma.

The Mercodia Proinsulin ELISA is a highly specific tool registered for in vitro diagnostics (IVD) use. It is calibrated against International Reference Reagent IRR 84/611 for human proinsulin. The specificity of the utilized antibodies enables measurement of human proinsulin without interference from insulin or C-peptide.

Each Mercodia Proinsulin ELISA kit contains reagents for 96 wells, sufficient for 42 samples and one calibrator curve in duplicate. The microplate strip format (12 strips x 8 wells) is ideal when only a limited number of samples need to be analyzed.

Mercodia Diabetes Antigen Control (10-1134-01 (US only) or 10-1164-01) is designed to be used as a two-level control for human proinsulin.

Test characteristics

Species：Human

Samples：Serum and plasma

Sample volume：50 µL

Assay range：3.3 - 130 pmol/L (0.03 - 1.18 µg/L)

Detection limit：≤1.7 pmol/L

Incubation：1 h + 1 h + 15 min

Detection method：Absorbance

specificity

The following cross-reactions have been tested:

Insulin：< 0.03 %

C-peptide：< 0.006 %

Proinsulin (Des 31-32)：95 %

Proinsulin (Split 32-33)：95 %

Proinsulin (Des 64-65)：84 %

Proinsulin (Split 65-66)：90 %

Porcine Proinsulin：4 %

Mouse Proinsulin I：3 %

Mouse Proinsulin II：16 %

Rat Proinsulin I：13 %

Rat Proinsulin II：10 %