Oxidized LDL ELISA

Measurement range: 1.00 - 25.0 mU/L (6.56 - 164 U/L when multiplied with dilution factor)

l  CE/IVD labeled on selected markets

l  Controls included in the kit

l  The unique specificity of antibody 4E6 enables measurement of all highly oxidized LDL, independent of other modifications of LDL

Description

The Mercodia Oxidized LDL ELISA kit is intended to be used for in vitro quantification of oxidized low-density lipoproteins (oxidized LDL) in human serum or plasma.

Mercodia Oxidized LDL ELISA is a laboratory assay used to quantify the levels of oxidized low-density lipoprotein, a central biomarker in atherosclerosis and cardiovascular disease.

Mercodia Oxidized LDL ELISA is a unique sandwich ELISA based on the proprietary mouse monoclonal antibody 4E6, which is directed against a conformational epitope in oxidized ApoB-100.

Elevated plasma levels of oxidized LDL are associated with higher cardiovascular risk and higher incidence of metabolic diseases, i.e. metabolic syndrome, obesity, and type 2 diabetes. Also, sub-clinical atherosclerosis, and kidney and liver diseases are associated with increased levels of oxidized LDL.

Test characteristics

Species：Human

Samples：Serum, plasma

Sample volume ：10 µL

Assay range：1.00-25.0 mU/L (6.56-164 U/L when multiplied with dilution factor)

Detection limit：≤ 0.5 mU/L

Incubation：2 h + 1 h + 15 min

Detection method：Absorbance

specificity

The following cross-reactions have been tested:

The proprietary mouse monoclonal antibody 4E6 is developed by professors Holvoet and Collen at the University of Leuven in Belgium. It is directed against a conformational epitope in the ApoB100 moiety of LDL that is generated as a consequence of substitution of at least 60 lysine residues of Apo B100 with aldehydes (Holvoet 2006). This number of substituted lysines corresponds to the minimal number required for scavenger-mediated uptake of oxidized LDL.

Substituting aldehydes can be produced by peroxidation of lipids of LDL, resulting in the generation of oxidized LDL. However, lipid peroxidation is not required. Indeed, aldehydes released by endothelial cells under oxidative stress or by activated platelets may also induce the oxidative modification of Apo B100 in the absence of lipid peroxidation of LDL.

The unique affinity of the antibody 4E6 used in the Mercodia Oxidized LDL ELISA makes it possible to measure both MDA and aldehyde-modified oxidized LDL, which sets this assay apart from assays based on other antibodies.