MPO ELISA

Measurement range: 4.0 - 200 μg/L

l  Broad measurement range

l  The MPO ELISA is calibrated against a highly purified, fully validated, commercially available MPO preparation

Description

The Mercodia MPO ELISA is a high quality enzyme immunoassay for the quantification of human myeloperoxidase (MPO) in plasma or serum.

Myeloperoxidase (MPO) is an iron-containing glycoprotein found in neutrophils and monocytes. It forms a tetrameric complex with a molecular weight of 150 kDa, composed of two glycosylated alpha chains (59-64 kDa) and two unglycosylated beta chains (14 kDa). Upon microbial invasion, MPO is released from neutrophil granules, converting hydrogen peroxide and chloride ions into a potent oxidizing agent called hypochlorous acid. MPO serves as a marker for airway inflammation in asthma and environmental irritant exposure. It’s also implicated in atherogenesis and a potential contributor to atherosclerosis. Elevated MPO levels in serum are linked to cardiovascular disease (CAD), making it valuable as an inflammatory marker to identify CAD patients at risk of cardiac events without myocardial necrosis.

Test characteristics

Species：Human

Samples：Plasma or serum

Sample volume：25 µL

Assay range：4.0 - 200 μg/L

Detection limit：≤ 3 μg/L

Incubation：1 h + 1 h + 15 min

Detection method：Absorbance

specificity

The following cross-reactions have been tested:

TPO：< 0.01 %

CRP：< 0.01 %

EPO：3.53 %

Lysosyme：0.03 %

Elastase：0.12 %

a 1-antitrypsin：< 0.01 %