Insulin (Total) MBeads Assay

Measurement range: 23.3–1302 mU/L

l  Low sample volume (5µL)

l  Short and easy protocol, no dilutions

l  For use in 96 and 384 well plates

l  Adapted for manual or automatic procedures

l  If required, a 96 well bio-magnetic plate can be purchased, requesting item 15-1000-01

Description

The Insulin (Total) MBeads Assay  is a high-throughput tool enabling high specificity results from small sample volumes.

Total Insulin Northern Lights® MBeads Assay is intended to be used for the measurement of insulin in conditioned media from perifusion experiments or other in vitro cultures from cells derived from human and mouse specimens. The assay allows the analysis of multiple samples simultaneously, decreasing the cost per data point and the amount of sample required. This enables the analysis of other analytes in volume-limited samples.

Read more: MBeads Brochure

Test characteristics

Species  Human and Mouse

Sample types  Cell media (perifusion samples, conditioned media)

Sample volume  5 µL

Detection limit  12.25 mU/L

Assay range  23.3–1302 mU/L

Incubation time  2 h + 10 min

Detection method  Chemiluminescence

specificity

The following cross-reactions have been tested:

C-peptide  ≤ 0.02 %

Proinsulin  59 %

Mouse insulin  68 %

Rat insulin  112 %