Insulin ELISA–10pack

Measurement range: 3 - 200 mU/L (0.13 - 8.70 µg/L)

l  Calibrated against 1st International Reference Preparation 66/304 for human insulin

l  No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)

l  Insignificant or no cross-reactivity to insulin analogs

l  CE/IVD labeled on select markets

l  Serum controls available: 10-1134-01 (US only) or 10-1164-01

Description

The Insulin ELISA – 10 pack is a high-quality Insulin ELISA for specific measurement of human insulin in serum, plasma or cell culture media.

The Mercodia Insulin ELISA has little or no cross-reactivity to insulin analogs, rat or mouse insulin, which enables distinguishing endogenous human insulin, both in patients undergoing treatment with insulin analogs, and in rat or mouse models.

Mercodia Diabetes Antigen Control (10-1134-01 (US only) or 10-1164-01) is designed to be used as a two-level control in the Insulin ELISA.

Test characteristics

Species：Human

Samples：Serum, plasma, cell culture media

Sample volume  25 µL

Assay range  3 - 200 mU/L

Detection limit  ≤ 1 mU/L (0.043 µg/L)

Incubation：1 h + 15 min

Detection method：Absorbance

Specificity

The following cross-reactions have been tested:

C-peptide：<0.01 %

Proinsulin：<0.01 %

Proinsulin des (31-32)：<0.5 %

Proinsulin split (32-33)：<0.5 %

Proinsulin des (64-65)：98 %

Proinsulin split (65-66)：56 %

Insulin aspart：4 %

Insulin detemir：Not detected

Insulin glargine：24 %

Insulin glulisine：Not detected

Insulin lispro：Not detected

IGF-I：<0.02 %

IGF-II：<0.02 %

Rat insulin：0.7 %

Mouse insulin：0.3 %

Porcine insulin：93 %

Ovine insulin：48 %

Bovine insulin：31 %