Lumi-Phos 530 ELISA AP Substrate

Lumi-Phos 530 is a ready-to-use chemiluminescent reagent formulation containing the alkaline phosphatase (AP) substrate, Lumigen PPD. Lumi-Phos 530 is useful for chemiluminescent detection of AP conjugates in immunoassays and DNA probe assays. It provides ultra-sensitive detection of analytes captured on magnetic particles and in microtiter plate wells.

l  Excellent sensitivity – 10,000 times more sensitive than colorimetric substrates in solution

l  High intensity light production – with low concentrations of AP the light intensity remains constant for several hours

l  Linear calibration curves – the luminescence intensity at any time point is a direct measure of the concentration of the enzyme

Reaction Mechanism

Solution Kinetic Profile

Chemiluminescent Enhancement

The unique co-surfactant system promotes energy-transfer to the fluorescent head groups causing the generation of luminescence with a broad maximum at 530 nm.

Product Specifications

Ordering Information