High Range Rat Insulin ELISA

Measurement range: 3 - 150 μg/L

l  No sample dilution for sample with high insulin concentrations

l  Results within 2.5 hours

l  Low sample volume - 10 µL

Description

Mercodia High Range Rat Insulin ELISA

A method for the quantitative determination of high levels of insulin in rat samples. The High Range Rat Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Mercodia Diabetes Antigen Control – Rat & Mouse (10-1220-01) is designed to be used as a three-level control for the Mercodia High Range Rat Insulin ELISA.

Test characteristics

Species：Rat

Sample types：Serum, EDTA plasma and cell culture medium.

Sample volume：10 µL

Assay range：3 - 150 μg/L

Detection limit：≤1.5 µg/L

Incubation：2 h + 15 min

specificity

The following cross-reactions have been tested:

Mouse insulin：75%

Rat C-peptide I：Not detected

Rat C-peptide II：Not detected

Mouse C-peptide I：Not detected

Mouse C-peptide II：Not detected

Rat Proinsulin I：8%

Rat Proinsulin II：51%

Mouse Proinsulin I：33%

Mouse Proinsulin II：51%

Human C-peptide：Not detected

Human insulin：167%

Human proinsulin：75%

Insulin lispro (Humalog® Eli Lilly)：167%