Ultrasensitive Rat Insulin ELISA

Measurement range: 0.02 - 1.0 μg/L

l  Very low detection limit - 0.02 µg/L

l  High specificity - Insignificant cross-reactivity to C-peptide and proinsulin

l  No interference from complex sample matrices

l  Controls available: 10-1220-01

Description

Mercodia Ultrasensitive Rat Insulin ELISA

A method for the quantitative determination of low levels of insulin in rat samples. The Rat Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Mercodia Diabetes Antigen Control – Rat & Mouse (10-1220-01) is designed to be used as a three-level control for the Mercodia Ultrasensitive Rat Insulin ELISA.

Test characteristics

Species：Rat

Samples types：Serum, EDTA plasma and cell culture medium.

Sample volume：25 µL

Assay range：0.02 - 1.0 μg/L

Detection limit：≤0.02 µg/L

Incubation：2 h + 30 min

Detection method：Absorbance

specificity

The following cross-reactions have been tested:

Mouse insulin：75 %

Rat proinsulin I：8 %

Rat proinsulin II：51 %

Mouse proinsulin I：31 %

Mouse proinsulin II：51 %

Mouse c-peptide I：Not detected

Mouse c-peptide II：Not detected

Rat c-peptide I  Not detected

Rat c-peptide II  Not detected

Human insulin  167 %

Human proinsulin  75 %

Human C-peptide：Not detected

Insulin lispro (Humalog®)：167 %