Total GLP-1 ELISA

Measurement range: 0.9 - 940 pmol/L (2.8 - 2940 pg/mL)

l  100% specificity to the predominating isoform, GLP-1 (9-36) amide

l  High sensitivity

l  Validated according to CLSI/FDA/EMA

l  Controls available: 10-1287-01

Description

Mercodia Total GLP-1 ELISA

The Mercodia Total GLP-1 ELISA provides a chemiluminescent method for the quantitative determination of amidated glucagon-like-peptide-1 (GLP-1) in human plasma and serum samples. The assay has been validated according to CLSI, FDA, and EMA guidelines.

GLP-1, is one of the major incretin hormones produced in the intestinal L-cells. It is secreted as the active forms (7-37)/(7-36) amide but is rapidly cleaved by the enzyme DPP4 forming the metabolites GLP-1 (9-37)/(9-36) amide. Although the two active forms have shown equal biological potency, the majority of circulating GLP-1 in humans is found in the amidated form.

Mercodia GLP-1 (9-36) Amide Control – Low, Medium, High (10-1287-01) is a three-level control for the Mercodia Total GLP-1 ELISA.

Test characteristics

Species：Human

Sample types：Serum and EDTA plasma

Sample volume：25 µL

Assay range：0.9 - 940 pmol/L (2.8 - 2940 pg/mL)

Detection limit：≤0.65 pmol/L

Incubation：2h + 15 min

Detection method：Chemiluminescence

specificity

The following cross-reactions have been tested:

GLP-1 (1-36) amide：88%

GLP-1 (7-36) amide：93%

GLP-1 (9-36) amide：100%

GLP-1 (1-37)：Not detected

GLP-1 (7-37)：Not detected

Glucagon：Not detected

GIP：Not detected

GLP-2：Not detected

Oxyntomodulin：Not detected

Glicentin：Not detected

Miniglucagon：Not detected

MPGF：Not detected

Liraglutide：Not detected

Exenatide：Not detected

Lixisenatide：Not detected

Dulaglutide：0.07%